Cloning of KCNE1 gene in long QT syndrome and construction of its eukaryotic expression vector
- VernacularTitle:先天性长QT综合征KCNE1基因的克隆及其真核表达载体的构建
- Author:
Wei LI
;
Junguo YANG
;
Rong DU
;
Qiumei XU
;
Siming GUAN
;
Qinmei KE
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
Long QT syndrome;
KCNE1 gene;
Clone;
Eukaryotic expression vector
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct the eurokaryotic expression vector of KCNE1 gene and express recombinant KCNE1 in HEK293 cells.Methods:Human KCNE1 gene fragment was amplified from human placenta total RNA by RT-PCR and cloned into the vector of pCR2.1 TOPO by means of T-A cloning.KCNE1 cDNA was obtained from pCR2.1-KCNE1 by restriction enzyme digestion and inserted into the same restriction site of pEGFP-N1.Thus pEGFP-N1-KCNE1 was constructed and transfected into HEK293 cells with Effectene transfection reagent.Results:The eukaryotic expression vector pEGFP-N1-KCNE1 was successfully constructed by gene cloning and recombinant method and expressed in HEK293 cells.Conclusion:The cloning of KCNE1 gene and the construction and expression of its eukaryotic expression vector may shed some light on further functional study of KCNE1 gene.
