Effect of CO_2 Pneumoperitoneum during Laparoscopy on Migration of GFP-labeled Living Cells into the Liver through Blood Route
- VernacularTitle:腹腔镜手术CO_2气腹对绿色荧光蛋白标记活细胞血路肝迁徙的影响
- Author:
Xihong YUAN
;
Kai YIN
;
Jun GAO
- Publication Type:Journal Article
- Keywords:
Pneumoperitoneum,artifical;
Animal model;
Green fluorescent protein;
Rat
- From:
Chinese Journal of Minimally Invasive Surgery
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of CO2 pneumoperitoneum on migration of GFP-labeled living cells into the liver through blood route in rat model.Methods SD rats was inoculated intraportally with high-dose(5?106) GFP-labeled liver cells from C57BL/6 mice after cutting belly open.Pneumoperitoneum was established immediately after closing the abdominal wall.The rats were randomly divided into four groups(n=10 in each) to receive CO2 pneumoperitoneum at 5,10,or 15 mmHg,or no treatment other than cells inoculation(control).The pneumoperitoneum was maintained for 30 min.Afterwards,the rats were euthanized by cervical dislocation,and the liver of the rats was removed for fast frozen section biopsy.The expression of GFP-labeled living cells in rat livers was compared between the groups.Results No significant difference was detected in the positive expression of GFP-labeled cells between the groups(8 rats in the control group,9 in the 5 mm Hg group,9 in the 10 mm Hg group,and 10 in the 15 mm Hg group,?2=2.222,P=0.528).The mean number of GFP-positive cells in the four groups was 6.63?2.45(control),7.67?2.83(5 mm Hg),13.89?4.37(10 mm Hg),and 15.50?6.29(15 mm Hg).There was significant differences between the four groups(F=10.78,P=0.000).In addition,the numbers of GFP-positive cells in the high pressure groups(15 mm Hg and 10 mm Hg) were significantly higher than that in the low pressure group(5 mm Hg) and the control(P
