Correlation of Hepatitis B Virus Basic Promoter Mutation with IL-10,IL-12,TNF-?,IFN-? as well as HBV DNA Contents in Chronic Hepatitis
- VernacularTitle:乙型肝炎病毒C基因启动子变异与白细胞介素10与12、肿瘤坏死因子?、?-干扰素及病毒含量的关系
- Author:
Liyi HUANG
;
Yanhong YU
;
Weijun XUAN
;
Jizhou WU
;
Lu HUANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Basic core promoter;
Gene mutation;
Interleukin-10;
Interleukin-12;
Tumor necrosis factor ?;
Interferon ?
- From:
Chinese Journal of Nosocomiology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the correlation of hepatitis B virus(HBV)basic core promoter(BCP) mutation with the interleukins(IL-10,IL-12),tumor necrosis factor(TNF)-?,interferon(IFN)-?,as well as HBV DNA contents in patients with chronic hepatitis B virus infection.METHODS(1) Project subject: 176 patients(chronic hepatitis B with mild,moderate and severe degree;liver cirrhosis,chronic fulminant and hepatocellular carcinoma(HCC)) with chronic hepatitis B virus infection were studied.(2) Project methods: ① The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined by the method of polymerase chain reaction(PCR) microplate hybridization ELISA in these patients.② The serum cytokines(IL-10,IL-12,TNF-? and IFN-?) of these patients were measured by specific-ELISA.RESULTS The serum levels of cytokines(IL-10(80.96?30.86 vs 72.11?24.19 mg/L),IL-12(41.33?15.10 vs 35.98?14.47 mg/L),TNF-?(56.04?27.05 vs 38.01?10.49 mg/L),IFN-?(19.81?12.29 vs 16.55?8.99 mg/L))and HBV DNA contents(108.2478?0.9826 vs 105.8876?1.4822copies/ml) in BCP mutant group were significantly higher than that in non-mutant group(P
