Trichosporon asahii cph1 Gene DNA Sequence Analysis
- VernacularTitle:阿萨希丝孢酵母cph1基因DNA序列分析
- Author:
Zhikuan XIA
;
Rongya YANG
;
Wenling WANG
;
Xin FAN
;
Congmin WANG
;
Xun ZHAO
- Publication Type:Journal Article
- Keywords:
Trichosporon asahii;
cph1;
Sequence analysis
- From:
Chinese Journal of Nosocomiology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To identify if Trichosporon asahii exist cph1 gene homolog of Candida albicans,and analyze its nucleotide sequence.METHODS Nuclear DNA was extracted from the cells of T.asahii by using a simplified protocol,designed 29 pairs of primers according to the cph1 gene sequence of C.albicans and amplify by PCR.The PCR products were cloned and sequenced using the ABI377 nucleotide sequenator,and BLAST analysis.RESULTS An 827 bp gene was amplified successfully,which was homolog with the cph1 gene of C.albicans and their identity was 97.3%.CONCLUSIONS This trial determines the clone cph1 gene in T.asahii for the first time,which makes bases for the role of cph1 gene in the hypha formation.
