Inhibition of growth of human neuroblastoma in nude mouse models by antisense VEGF_(165) cDNA transfection
- VernacularTitle:反义VEGF_(165)基因转染抑制裸鼠体内人神经母细胞瘤的生长
- Author:
Mingjie WEN
;
Shentao LI
;
Yamei WANG
;
Yahui QI
- Publication Type:Journal Article
- Keywords:
vascular endothelial growth factor;
antisense;
neuroblastoma;
microvessel density;
ultrastructure
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitive effect of antisense VEGF165 cDNA on growth and angiogensis of human neuroblastoma in nude mouse models.Methods Three kinds of SH-SY5Y cells which had been stably transfected by sense VEGF165 cDNA,antisense VEGF165 cDNA and empty vector respectively,were inoculated subcutaneously to nude mice.The size of subcutaneous tumors was measured,and the morphology of tumor tissue was observed by microscope.The microvessel density(MVD)in tumor mass was analyzed by CD31 immunohistochemistry staining.Apoptotic cells were detected by the electron microscope.Results The growth of tumor mass,which was inoculated with antisense VEGF,significantly decreased in nude mice compared with empty vector group(P
