Effect of Herceptin on apoptosis and cell cycle in breast cancer cell line SKBR-3
- VernacularTitle:赫赛汀诱导乳腺癌细胞凋亡及对其细胞周期的影响
- Author:
Hongzhi LI
;
Yi ZHENG
;
Peng LIN
;
Changming DOU
;
Jieying DONG
;
Tongwen WANG
- Publication Type:Journal Article
- Keywords:
Herceptin;
breast cancer;
apoptosis;
HER-2;
cell cycle
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of an immuno-targeted therapy drug Herceptin on apoptosis and cell cycle of overexpressing HER2 breast cancer cells.Methods Breast cancer cell line SKBR-3 was treated with Herceptin at most effective dosage and exposure time were selected by MTT assay.Then the apoptotic features,the apoptotic rate and the change of cell cycle of breast cancer cells were detected by fluorescent microscope(FM),laser confocal microscope(LCM),scanning electron microscope(SEM),transmission electron microscope(TEM)and flow cytometry(FCM).Results With the treatment of Herceptin,the visible apoptosis features of SKBR-3 cells were observed by FM,LCM,SEM and TEM.Compared to the control cell group,the rate of initial apoptotic cells detected by FCM with Annexin V/PI staining increased significantly(P
