Comparison of expression of anticoagulant factors by vascular endothelial-like cells derived from BMMSCs and from HUVEC
- VernacularTitle:骨髓间充质细胞来源的血管内皮样细胞与脐静脉血管内皮细胞抗凝基因表达的比较
- Author:
Xiaozheng LIU
;
Zhijun HAN
;
Hua REN
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
vascular endothelial cells;
anticoagulant gene;
gene expression;
bone marrow
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the difference in anticoagulant ability of endothelial cells from different sources.Methods Bone marrow mesenchymal stem cells(BMMSCs)were cultured,purified,and expanded by Ficoll-Paque density gradient centrifugation and adherent culture in vitro.Then they were induced and differentiated in medium with 10 ?g/L VEGF.After 7 days,Von Willebrand factor(VWF)of the cells were identified by immunohistochemistry.At last,the major anticoagulant gene expression of the vascular endothelial-like cells derived from BMMSCs and the human umbilical vein endothelial cells(HUVECs)was detected and compared by reverse transcriptase PCR(RT-PCR).Results Though BMMSCs can successfully differentiate into vascular endothelial-like cells in vitro,they fail to express mRNA of the major anticoagulant gene.However,HUVECs can express the mRNA of these genes.Conclusion BMMSCs can differentiate into vascular endothelial-like cells in vitro,but their anticoagulant ability is inferior to HUVECs.
