Methodological evaluation on ELISA for detecting concentration of apo A-V in human plasma
- VernacularTitle:ELISA法测定人血清中apo AⅤ浓度
- Author:
Min HU
;
Shuiping ZHAO
;
Tao ZHANG
;
Yi PAN
- Publication Type:Journal Article
- Keywords:
apo A-V;
ELISA;
methodology
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an ELISA to detect the concentration of apo A-V in human plasma and evaluate the sensitivity and stability of the method.Methods The standard curve of recombinant human apo A-V, the lowest detectable concentration, the day-to-day variation and between-plate-within-day variation, the interference of other non-specialized apolipoprotein were examined, and the reference range was established by detecting the plasma concentration of apo A-V in 505 healthy subjects.Results The linear range of the established method was 5.86 - 187.5 ng/ml with lowest detectable concentration of 2.34 ng/ml. The interferences for apo A-V detection due to Lp(a), apo AI and apo B was never found in our study. The day-to-day variation and between-plate-within-day variation were less than 10% respectively.Conclusion An ELISA for detecting the concentration of apo A-V in human plasma was established and the method can serve as a useful tool for research on mechanism and function of apo A-V in TG metabolism.
