Effect of threat stress on expression of GnRH receptor in stomach of Sprague-Dawley (SD) rats
- VernacularTitle:恐吓应激对SD大鼠胃GnRH受体表达的影响
- Author:
Meijuan HUANG
;
Zhu HUANG
;
Chao JIANG
;
Huiru XU
;
Chenyang WANG
;
Lin HOU
;
Bing YAO
- Publication Type:Journal Article
- Keywords:
threat;
stress;
stomach;
GnRH receptor;
rat
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the localizations and the quantity of GnRH receptor in stomach of Sprague-Dawley (SD) rats under stress.Methods The model of stress SD rats was established by fear. Then, stomachs were taken from the rats in acute stress group (2h-12h), chronic stress group (1d-4w) and the control group respectively.The localizations and the quantity of GnRH receptor in stomachs were detected using immunohistochemistry and Western blotting.Results The results of immunohistochemistry showed that immunoreactivity of GnRH receptor was displayed in the gastric parietal cells and the epithelial cells of the gastric pits in stomachs of rats in all groups. The immunoreactive materials were distributed in membrane and cytoplasm of all positive cells, but not in nuclei. Meanwhile, the results of Western blotting showed that the number of GnRH receptor decreased significantly when SD rats were in stress from 2h to 2w (P
