Effect of ATP sensitive potassium channel opener on neuronal apoptosis and the expression of caspase-8 in rats following cerebral ischemia-reperfusion
- VernacularTitle:三磷酸腺苷敏感性钾通道开放剂对大鼠脑缺血再灌注后神经元凋亡及天冬氨酸特异性半胱氨酸蛋白酶-8表达的影响
- Author:
Hong ZHANG
;
Ying MA
;
Yanyan LIU
- Publication Type:Journal Article
- Keywords:
cerebral ischemia;
apoptosis;
ATP sensitive potassium channel;
opener;
caspase-8
- From:
Journal of Clinical Neurology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of ATP sensitive potassium channel(KATP)opener on neuronal apoptosis and the expression of caspase-8 mRNA and protein in rats following cerebral ischemia-reperfusion.Methods 200 male Wistar rats were randomly divided into sham-operated group,control group,KATP opener precondition group(KATP opener group)and KATP opener and blocker precondition group(KATP blocker group).The middle cerebral artery ischemia-reperfusion models were established by using the intraluminal suture occlusion method.Neuronal apoptosis were detected by TUNEL staining,the expression of caspase-8 mRNA and protein were detected by immunohistochemical staining and RT-PCR methods.Results(1)The numbers of apoptotic cells in KATP opener group at 12 h,24 h,48 h,72 h after cerebral ischemia-reperfusion were significantly less than those in control group and KATP blocker group(P
