Expression and correlation between erythropoietin mRNA and hypoxia inducible factor-1? mRNA in rats with brain ischemic tolerance
- VernacularTitle:脑缺血耐受大鼠促红细胞生成素和缺氧诱导因子-1?的表达及其相关性
- Author:
Zhongling SUN
;
Renliang ZHAO
;
Ruijian DONG
- Publication Type:Journal Article
- Keywords:
ischemic preconditioning;
ischemic tolerance;
cerebral ischemia;
erythropoietin;
hypoxia inducible factor-1?
- From:
Journal of Clinical Neurology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the expression and correlation between erythropoietin(EPO)mRNA and hypoxia inducible factor-1?(HIF-1?)mRNA in rats with brain ischemic tolerance induced by ischemic preconditioning.Methods Models of cerebral ischemic preconditioning and ischemic-reperfusion(preconditioning group)were made by twice suture method,after the short ischemic preconditioning 1 d,3 d,7 d,14 d and 21 d respectively,the models were underwent ischemic-reperfusion again.The infarct volume was measured by tetrazolium chloride(TTC)staining,the expressions of HIF-1? mRNA and EPO mRNA in the different time(1～21 d)of ischemic-reperfusion were detected by reverse transcriptase polymerase chain reaction(RT-PCR)and compared with non-preconditioning group.Results The infarct volume in the 1 d,3 d and 7 d subgroups of preconditioning group were significantly smaller compared with non-preconditioning group(all P
