Different concentrations of glycyrrhizin on hepatic stellate cell proliferation and its control mechanism

VernacularTitle:不同浓度甘草甜素对肝星状细胞增殖的影响及其调控机制的研究
Author: Wenyan QIN ; Yonghua CHEN ; Qunfen CAO ; Qiuyan JIANG
Publication Type:Journal Article
Keywords: GLYCYRRHIZIN△/AD; Hepatocytes/DE/PA; Cell proliferation/DE
From: Journal of Chinese Physician 2011;13(4):464-466,470
CountryChina
Language:Chinese
Abstract: Objective To explore the different concentrations of glycyrrhizin on hepatic stellate cell proliferation and its regulation mechanism.MethodsHepatic stellate cell proliferation rate of three different concentrations of glycyrrhizin was detected by MTT assay.The expression of cyclin E was detected by immunocytochemistry.ResultsMTT assay was used to detect the cell proliferation inhabitation in different concentration groups,from control group 0.8 mg/L glycyrrhizin group to 1.2 mg/L glycyrrhizin group and 1.6 mg/L glycyrrhizin group.The cell proliferation inhibited rates were 0,2.73%,14.75 % and 25.96%.The difference was significant (P＜0.05).The numbers of Cyclin E positive cells in different concentrations of glycyrrhizin group were significantly different compared with control group,and it was concentration dependently decreased (P＜0.01).ConclusionsGlycyrrhizin concentration dependently inhibited rat hepatic stellate cells proliferation and it could reduce the expression of cyclin E and inhibit the proliferation of rat hepatic stellate cells.