Why is it difficult for PCR-SSP to determine some alleles at HLA-B locus?
- VernacularTitle:B~* 48与B~* 1568难以应用SSP方法确定低分辨分型的分析
- Author:
Kourong MIAO
;
Qinqin PAN
;
Min XUE
- Publication Type:Journal Article
- Keywords:
Allele;
HLA;
Sequence based typing (SBT);
Sub-cloning
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
CG. And that was why SSP failed to determine the allele.Conclusion The difficulty in HLA genotyping by SSP resulted from the primers, which involved unknown sequence of Exon 1 at locus B in the studied sample.