Expression of ?-Lactamase in Pseudomonas aeruginosa Biofilms
- VernacularTitle:生物膜铜绿假单胞菌产?-内酰胺酶的实验研究
- Author:
Qiang WANG
;
Handong JIANG
;
Jie CHAI
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Biofilm;
?-Lactamase;
AmpC ?-lactamase;
Imipenem;
Ceftazidime
- From:
Chinese Journal of Nosocomiology
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore production of ?-lactamase induced by antibiotics in Pseudomonas aeruginosa biofilm and planktonic bacteria.METHODS In vitro models of P.aeruginosa biofilm were built up.The planktonic bacteria and P.aeruginosa biofilms were exposed to different concentrations of imipenem and ceftazidime.The quantitative analysis of ?-lactamase was undertaken.The class of ?-lactamase was verified by modified cefoxitin three-dimensional test.RESULTS Both P.aeruginosa biofilm and planktonic bacteria showed significant ?-lactamase activity in the presence of antibiotics.The maximal ?-lactamase activities of both were significantly different with the statistic method of test(P
