Construction of Yeast Two-hybrid Genomic Library of Rhodococcus equi
- VernacularTitle:马红球菌酵母双杂交基因组文库的构建
- Author:
Jiyong YANG
;
Dingxia SHEN
- Publication Type:Journal Article
- Keywords:
Rhodococcus equi;
Yeast two hybrid;
Library
- From:
Chinese Journal of Nosocomiology
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To construct two-hybrid genomic library of Rhodococcus equi,and study the pathogenic mechanism of R.equi.METHODS The genomic DNA of R.equi was partially digested and cloned into AD vector of two-hybrid system,the number of independent clone and the size of insert fragment were detected as the quality index of the library.RESULTS The independent clone of the library was 1?106 and the average size of insert fragment was 2.3 kb.CONCLUSIONS The pathogenic mechanism of R.equi is not clear and it is very important for this study to construct two-hybrid genomic library.
