Study on the relationship of PTEN mRNA expression and Akt phospholylation in acute leukemia
- VernacularTitle:急性白血病PTEN mRNA表达和Akt磷酸化水平的相关性研究
- Author:
Boli LI
;
Baoguo CHEN
;
Wenda LUO
;
Liping MEI
;
Weihua YAN
;
Min ZHU
;
Huihui XU
- Publication Type:Journal Article
- Keywords:
Leukemia;
Genes;
Flow cytometry;
Phospholylation
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between PTEN mRNA expression and Akt phospholylation in acute leukemia(AL)and explore its clinical significance in the development of acute leukemia.Methods PTEN mRNA expression and Akt phospholylation level in the leukemia cell line K562, HL-60,Jurkat,21 healthy persons and 68 patients with AL were analyzed with RT-PCR and Flow cytometry, respectively.Results(1)The significantly different PTEN mRNA expression was found between the controls and AL patients.85.70% of the normal controls and 26.47% AL patients show positive respectively (?~2=23.38,P
