Effects of mitogen-activated protein kinase p38 on CD3/CD28-stimulated T-cell proliferation and IL-2 expression enhauced by shockwaves
- VernacularTitle:p38 MAPK对冲击波促进T淋巴细胞增殖和分泌IL-2的作用
- Author:
Yi ZHAO
;
Tiecheng YU
;
An JIN
;
Jianguo LIU
;
Xueqing ZHENG
;
Xinxiang XU
- Publication Type:Journal Article
- Keywords:
shockwaves;
T cell proliferation;
interleukin 2;
mitogen-activated protein kinase activating p38
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mitogen-activated protein kinase p38(p38 MAPK)on CD3/CD28-stimulated T-cell proliferation and IL-2 expression which were enhanced by shockwaves.Methods Jurkat T cells or peripheral blood mononuclear cells(PBMC)were pretreated with the p38 MAPK-inhibitor(SB203580)(The Jurkat T cells or PBMC of the control groups were not pretreated with SB203580),then treated with shockwaves,and stimulated respectively with a suboptimal dose of anti-CD3 and anti-CD28 antibodies or PHA.Finally the IL-2 productions of Jurkat T cells or the proliferation of PBMC were respectively measured.The protein tyrosine phosphorylation of p38 MAPKin Jurkat T cells treated either with shockwaves or not was measured by Western blotting with anti-phosphotyrine antibodies(Thr180/Tyr182).The expressoins of p38 MAPK in Jurkat T cells treated either with shockwaves or not were determined by Western blotting with anti-p38 MAPK antibodies.Results Compared with negative control group without shockwave treatment,the 3H-TdR incorporation of the phytohemagglutinin-stimulated PBMC,which were treated with low dose shockwaves(LDSWs)of 100,150,200,250,300,330 impulses at(0.180?0.015)mJ?mm2,increased significantly(P
