Differentiation of PC12 cells into neurons induced by NGF
- VernacularTitle:NGF诱导PC12细胞向神经元的分化
- Author:
Xiuli WANG
;
Dong CHEN
;
Jiamei LIU
- Publication Type:Journal Article
- Keywords:
PC12 cells;
nerve growth factor;
differentiation
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the condition of pheochromocytoma cells(PC12 cells)differentiation into neurons induced by nerve growth factor(NGF).Methods There were five groups :10,20,50,and 80 ?g?L-1 NGF groups and control group which contained 10% fetal calf serum.PC12 cells were exposed to NGF with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h,respectively.The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to NGF for 72 h.Results The number of MAP2 positive cells was significantly increased in 20,50,and 80 ?g?L-1 groups compared with control group and the most significant concentration was 50 ?g?L-1(P
