Effects of pancreatic kininogenase on expression of TGF-?_1 and collagen remodeling in spontaneously hypertensive rats
- VernacularTitle:胰激肽原酶对自发性高血压大鼠心肌转化生长因子-?_1表达及胶原重构的影响
- Author:
Jiyan LENG
;
Yanqiu YANG
;
Jun FU
;
Bushang LIU
- Publication Type:Journal Article
- Keywords:
kallidin/therapeutical use;
TGF-?1;
collagen remodeling;
rats,inbred SHR
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effects of pancreatic kininogenase on the expression of TGF-?1 and collagen remodeling in myocardiom of spontaneously hypertensive rats(SHR).Methods Twenty-four male SHR(aged fifteen weeks)were randomly divided into three groups:SHR group,pancreatic kininogenase group and captopril group(n=8),8 male Wistar Kyoto rats with normol blood pressure was considered as control group.Pancreatic kininogenase was given by peritoneal injection(7.2 U? kg-1? d-1),captopril was given by intragastric administration(10 mg? kg-1? d-1),the rats in SHR group and control group were administered with 0.9% NaCl(2 mL ? kg-1? d-1)through peritoneal injection.After four-week experiment,the pressure was measured in rats througth carotid artery,the rats were sacrificed and left ventricular mass index,collagen volume fraction,peripheral vascular collagen area were measured.Myocardial tissue was stained with VG and pathological changes were observed.The expression of TGF-?1 were detected by immunohistochemical technique(SP method).Results The systolic blood pressure,left ventricular mass index,collagen volume fraction,peripheral vascular collagen area and the expression level of TGF-?1 in SHR group were obviously higher than those in control group(P0.05).Conclusion Pancreatic kininogenase can obviously control pressure and reverse myocardial fibrosis probably by decreasing the expression of TGF-?1 in SHR.
