Establishment of cell model of Parkinson's disease and toxic effect of rotenone on dopaminergic neurons
- VernacularTitle:帕金森病细胞模型的建立及鱼藤酮对多巴胺能神经元的毒性作用
- Author:
Haina ZHANG
;
Guohua HU
;
Qiuhui CHEN
;
Dong SUN
;
Caixia LIU
;
Yajuan SUN
- Publication Type:Journal Article
- Keywords:
rotenone;
PC12;
?-synuclein;
apoptosis;
Parkinson's disease
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish cell model of Parkinson's disease(PD)and to approach the toxic effect of rotenone on dopaminergic neurons and its mechanism.Methods PC12 cells were differentiated into dopaminergic neurons and treated with various concentrations of rotenone.The morphological changes of PC12 cells were observed after treated with rotenone(0,10,25,50,75,and 100 nmol?L-1)for 24,48 and 72 h,the cell viability was measured by MTT assay.Immunohistochemistry and immunofluorescence were used to observe the accumulation of ?-synuclein in cytoplasm.AO/EB double staining was also adopted to test apoptosis.Results After differentiation PC12 cells shaped irregularly with big and long ecptomas and multiple cell conjunctions.After the treatment of rotenone cell ecptomas vanished gradually and cell bodies became smaller and smoother.The cell viability began to decline significantly at a concentration of 50 nmol?L-1 for 24 h(P
