Effect of aspirin and celecoxib on proliferation of breast cancer cell line MCF-7
- VernacularTitle:阿司匹林和塞来昔布对乳腺癌细胞MCF-7增殖的影响
- Author:
Yan ZHANG
;
Yang WANG
;
Xiuxin YAN
- Publication Type:Journal Article
- Keywords:
nonsteroidal antiinflammatory drugs;
aspirin;
celecoxib;
anastrozole;
breast neoplasms;
human breast cancer cell;
MCF-7
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss and compare the anti-tumor effects of aspirin and celecoxib on breast cancer cell MCF-7 through investigating the effects of aspirin,celecoxib,and combined with anastrozole respectively on the growth of human breast cancer cell MCF-7.Methods The human breast cancer cell MCF-7 were treated with 2.5,5.0,and 10.0 mmol?L-1 aspirin and 30,60,and 120 ?mol?L-1 celecoxib for 24,48,and 72 h respectively,the MCF-7 without treatment with drug was used as negative control group,the MCF-7 treated by ADM was used as positive control group,the inhibitory effect was detected by MTT assay.Besides,the MCF-7 cells were treated by anastrozole(0.5 and 1.0 ?mol?L-1),anastrozole(0.5 and 1.0 ?mol?L-1)combined with aspirin(2.5,5.0,and 10.0 mmol?L-1)or celecoxib(30,60,and 120 ?mol?L-1)for 48 h,respectively,the inhibitory rate was detected by MTT assay.Results ① The inhibitory rate of the MCF-7 cell line treated with aspirin was reduced compared with controls,which was in time-dependent and dose-dependent manner(P
