p38 mitogen-activated Protein Kinase in Vascular Smooth Muscle Cells Proliferation in SHR
- VernacularTitle:p38丝裂素活化蛋白激酶介导自发性高血压大鼠血管平滑肌细胞增殖
- Author:
Liuqing XU
;
Qiong YE
;
Changsheng XU
;
Huajun WANG
;
Kegui WU
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinase;
Angiotensin Ⅱ;
Platelet-derived growth factor;
Cell proliferation;
Vascular smooth muscle cell
- From:
Chinese Journal of Hypertension
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of p38 mitogen-activated protein kinase(p38MAPK) pathway in the proliferation of SHR vascular smooth muscle cells(VSMC) induced by angiotensin Ⅱ(Ang Ⅱ) and platelet-derived growth factor-BB(PDGF-BB).Methods VSMC were obtained from SHR thoracic aorta.DNA synthesis was quantified by measuring [3H]-thymidine incorporation.The p38MAPK activity was evaluated by immunobloting technique with phospho-p38MAPK antibody.Results 1) Ang Ⅱ(10-8-10-6 mol/L),PDGF(3-30 ng/L) dose-dependently increased the proliferation activity of VSMC([3H]-TdR incorporation).Ang Ⅱ(10-7 mol/L) or PDGF(10 ng/L) significantly increased [3H]-TdR incorporation rate in VSMCs(Ang Ⅱ:11 588?1322 vs control 2546?207 counts/min,P
