Effects of natural raw meal (NRM) on high-fat diet and dextran sulfate sodium (DSS)-induced ulcerative colitis in C57BL/6J mice.
- Author:
Sung Ho SHIN
1
;
Jia Le SONG
;
Myoung Gyu PARK
;
Mi Hyun PARK
;
Sung Joo HWANG
;
Kun Young PARK
Author Information
- Publication Type:Original Article
- Keywords: Natural raw meal (NRM); anti-inflammation; cytokines; iNOS; COX-2
- MeSH: Adiponectin; Animals; Biomarkers; Body Weight; Cholesterol; Colitis; Colitis, Ulcerative*; Colon; Cyclooxygenase 2; Cytokines; Dextran Sulfate*; Dextrans*; Diet, High-Fat*; Edema; Enzyme-Linked Immunosorbent Assay; Eosine Yellowish-(YS); Hematoxylin; Insulin; Interleukin-6; Interleukins; Leptin; Lipoproteins; Meals*; Mice*; Mucous Membrane; Nitric Oxide Synthase Type II; Obesity; RNA, Messenger; Triglycerides; Tumor Necrosis Factor-alpha; Ulcer*
- From:Nutrition Research and Practice 2015;9(6):619-627
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/OBJECTIVES: Colitis is a serious health problem, and chronic obesity is associated with the progression of colitis. The aim of this study was to determine the effects of natural raw meal (NRM) on high-fat diet (HFD, 45%) and dextran sulfate sodium (DSS, 2% w/v)-induced colitis in C57BL/6J mice. MATERIALS/METHODS: Body weight, colon length, and colon weight-to-length ratio, were measured directly. Serum levels of obesity-related biomarkers, triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), insulin, leptin, and adiponectin were determined using commercial kits. Serum levels of pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, and IL-6 were detected using a commercial ELISA kit. Histological study was performed using a hematoxylin and eosin (H&E) staining assay. Colonic mRNA expressions of TNF-alpha, IL-1beta, IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were determined by RT-PCR assay. RESULTS: Body weight and obesity-related biomarkers (TG, TC, LDL, HDL, insulin, leptin, and adiponectin) were regulated and obesity was prevented in NRM treated mice. NRM significantly suppressed colon shortening and reduced colon weight-to-length ratio in HFD+DSS induced colitis in C57BL/6J mice (P < 0.05). Histological observations suggested that NRM reduced edema, mucosal damage, and the loss of crypts induced by HFD and DSS. In addition, NRM decreased the serum levels of pro-inflammatory cytokines, TNF-alpha, IL-1beta, and IL-6 and inhibited the mRNA expressions of these cytokines, and iNOS and COX-2 in colon mucosa (P < 0.05). CONCLUSION: The results suggest that NRM has an anti-inflammatory effect against HFD and DSS-induced colitis in mice, and that these effects are due to the amelioration of HFD and/or DSS-induced inflammatory reactions.
