The Expression of HSP induced by Hantavirus in Vero-E6 cells in vitro
- VernacularTitle:汉坦病毒体外感染Vero-E6细胞诱导多种热休克蛋白的表达
- Author:
Lu YU
;
Heng MA
;
Chunguang DUAN
;
Shoujing YANG
- Publication Type:Journal Article
- Keywords:
Hantavirus;
Heat shock protein;
Hantavirus nucleocapsid protein
- From:
Journal of Medical Postgraduates
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study heat shock proteins(HSP) and their association with Hantavirus nucleocapsid protein(HV-NP) in Vero-E6 cells infected with Hantaan76-118.Methods: The HV-NP was identified by immunocytochemical staining after infected with Hantaan76-118.The expression of HSP was detected by Western-blot and analyzed by double specific antibody sandwich ELISA.Results: Western-blot exhibited that the expressions of HSP27,HSP70 and Grp94 in the Vero-E6 cells infected with Hantaan 76-118 were significantly higher than those in the control cells(P
