NRSF Plays a Regulatory Role in Human Insulin Gene Transcription
- VernacularTitle:神经元限制性沉默因子对人胰岛素基因转录调控作用的研究
- Author:
Qingbin LIU
;
Yanhua LI
;
Yinxiang YANG
;
Hongfeng YUAN
;
Rui ZHANG
;
Lipeng QIN
;
Yang L
;
Sainan ZHANG
;
Dijin SUN
;
Lin CHEN
;
Cixian BAI
;
Xue NAN
;
Yunfang WANG
;
Xuetao PEI
- Publication Type:Journal Article
- Keywords:
neuronal restrictive silencer factor(NRSF) ,neuronal restrictive silencer element(NRSE) ,insulin,promoter
- From:
Progress in Biochemistry and Biophysics
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The transcriptional repressor RE1 silencer transcription factor(NRSF/REST) is an important factor that restricts some neuronal traits in neurons.Since these traits are also present in pancreatic islet cells,NRSF-regulated genes involved in islet function are searched.A NRSE-like motif was analysed in human insulin promoter.The role of NRSE was evaluated by generating a model of insulin-secreting cells that firmly express NRSF.The presence of NRSF led to a decrease in activity of human insulin promoter by stable or transient transfection with human insulin-promoter luciferase.The predicted NRSE-like motif also confers NRSF-dependent transcriptional repression in the context of a surrogate gene promoter.Specific binding activity of NRSF/REST to the NRSE-like motif was confirmed by EMSA.Moreover,the binding activity is competed by consensus NRSE sequence.These data showed that human insulin promoter is regulated by the transcriptional repressor NRSF/REST via the NRSE-like motif.
