Meticillin Resistance and Glycopeptide Resistance in Clinical Coagulase-negative Staphylococci:Detection and Analysis
- VernacularTitle:耐甲氧西林凝固酶阴性葡萄球菌及糖肽类耐药的检测与分析
- Author:
Yuanchun ZHANG
;
Shuang LI
;
Fenglian LI
;
Yingchun LIU
;
Jing WANG
;
Ping WANG
- Publication Type:Journal Article
- Keywords:
Coagulase-negative staphylococci;
Meticillin-resistant coagulase-negative staphylococci;
mecA;
van;
Staphylococci with heteroresistance to vancomycin
- From:
Chinese Journal of Nosocomiology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide laboratory evidence for the prevention and control of coagulase-negative staphylococci(CNS) and study the prevalence of meticillin resistance and vancomycin resistance in clinical CNS in our hospital.METHODS Meticillin-resistant coagulase-negative staphylococci(MRCNS) were detected with cefoxitin disk diffusion and mecA-PCR.CNS with reduced susceptibilities to vancomycin was detected with vancomycin agar screen test.Their MIC was determined with E-test and gene van was detected with multiplex PCR.RESULTS CNS was highly resistant to cephem,?-lactam,aminoglycoside,macrolide,and lincosamide.The confirm rate of cefoxitin disk diffusion to mecA-PCR was 96.2% and 92.3% of mecA in 52 CNS strains were detected.Four strains of staphylococci with heteroresistance to vancomycin(VRS) were screened in 256 CNS ones which were all MRCNS but without gene van detected.CONCLUSIONS It is important for clinical laboratory to detect VRS and MRCNS with suitable methods to prevent VRS infection and prevalence.
