Dengue Type Ⅰ Virus Isolated in Guangzhou Detected by Using TaqMan MGB Real-time PCR
- VernacularTitle:TaqMan MGB实时荧光定量PCR检测登革Ⅰ型病毒
- Author:
Zhaofan LUO
;
Hongman XUE
;
Jianwei LIU
;
Wenzhong ZHAO
- Publication Type:Journal Article
- Keywords:
Dengue type 1 virus;
Real-time PCR;
Detection
- From:
Chinese Journal of Nosocomiology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To detect serotype of dengue virus in sera from patients with fever.METHODS A pair of degenerated primers and one MGB probe were designed targeting the conserved region at the E gene of dengue type 1 virus.TaqMan MGB real-time PCR assay was developed with plasmid including E gene of dengue type 1 virus as standard sample.The sera of 10 patients with fever were used to extract RNA,and convert into cDNA.Then cDNA were detected by TaqMan MGB real-time PCR assay and the amplified products were analyzed at the same time.RESULTS The sera of 9 patients from 10 samples were observed to generate a fluorescent signal,and about 100 bp fragment was obtained simultaneously.CONCLUSIONS Dengue fever on 2006 in Guangzhou is caused by the dengue type 1 virus.TaqMan MGB real-time PCR assay is rapid and sensitive to detect dengue virus infections.
