Cytokines secretion by splenocytes of mice was promoted by recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis
- VernacularTitle:多房棘球绦虫重组BCG-EmⅡ/3疫苗促进小鼠脾细胞因子的分泌
- Author:
Wengui LI
;
Hong WANG
;
Youming ZHU
- Publication Type:Journal Article
- Keywords:
Echinococcus multilocularis;
recombinant BCG-EmII/3 vaccine;
cytokines
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To dynamically observe changes of cytokines of splenocytes in mice by immunization with recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis(Em).Methods BALB/C mice were intranasally vaccinated and then killed to get spleen on 0、2、4、6、8 and 10w of immunization respectively, splenocytes were isolated to culture with stimulation of EmAg or ConA,their supernatants were gathered to measure the level of IL-2,IFN-?,TNF-? and IL-4 by ELISA Kits. Results In the groups of immunization, levels of IL-2,IFN-?,TNF-? and IL-4 obviously increased on 2～6、2～6、2～10 and 8～10w respectively, reached the highest level on 4、4、8 and 10w respectively. Conclusion TH1 response was induced in mice by rBCG-EmII/3 vaccine by early immunization(2～8w).
