Single nucleotide polymorphism of human APM1 detected by using allelic 3′-terminal specific primers real-time PCR
- VernacularTitle:等位基因3′末端碱基特异性引物定量PCR检测人脂联素基因单核苷酸多态性
- Author:
Xianrang SONG
;
Shufang WANG
;
Weiling CHI
- Publication Type:Journal Article
- Keywords:
human adiponectin;
single nucleotide polymorphism;
real-time PCR
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an analyzing technique of single nucleotide polymorphism(SNP) based on non-probe real-time quantitative PCR, and to explore the relationship between SNP in human adiponectin (APM1) gene and type 2 diabetes (T2DM).Methods Design primers with specific 3′-terminal nucleotides and perform Real-time PCR using SYBR Green dye. Genotypes were determined by comparing the amplification efficiency of each pair of primer and verified by sequencing. SNPs 45 and 276 in APM1 of 20 T2DM, 24 obesity and 28 healthy persons were analyzed. Results This technique could discriminate genotype as efficient as sequencing. The genotype of APM1 at +45 site was significantly correlated with T2DM (P
