Application of multiplex amplification of STRs for monitoring survival of engraftment after bone marrow transplantation
- VernacularTitle:用荧光标记短串连重复复合扩增技术监测骨髓移植存活
- Author:
Yi YE
;
Jin WU
;
Yingbi LI
- Publication Type:Journal Article
- Keywords:
multiplex amplification;
bone marrow transplantation;
chimera;
short tandem repeat(STR)
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of fluorescent multiplex amplification of short tandem repeat(STR)for monitoring survival of engraftment after bone marrow transplantation(BMT).Methods Three STR loci named D12S391,D18S865 and D20S161 in 56 cases were detected by fluorescent multiplex amplification.PCR products were separated and typed by DNA Sequencer.Results The genotypes of STR in 52 recipient after bone marrow transplantation were completely identical with those of the donors.In another 4 cases the evidences of mixed chimerism were observed.Conclusion The system of fluorescent multiplex amplification of STRs exhibited high capacity of discrimination and low cost.Its application in the detection of STR after BMT is reliable,sensitive and simple.Combined with the clinical manifestation it can be used to evaluate the effect of BMT.
