Cloning and Construction of Nucleic Acid Vaccine of FABP Gene cDNA from Echinococcus granulosus
- VernacularTitle:细粒棘球蚴内蒙株FABP基因cDNA的克隆与核酸疫苗的构建
- Author:
Huifang HAO
;
Zhigang WANG
;
Zhiwei LI
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Fatty acid binding protein(FABP);
Nucleic acid vaccine
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Specific primers were designed according to published nucleotide sequence of FABP (fatty acid binding protein) gene in the GenBank database. The kozak sequence (CCACC) was introduced at the upstream of initiator. The total RNA was extracted from protoscoleces of Echinococcus granulosus (Inner Mongol isolate). The FABP gene cDNA fragment was amplified by RT-PCR and cloned into pMD19-T vector for sequencing and analyzing. The cloned FABP gene cDNA was with 402bp. The ORF encoded 133 amino acids. The amplified cDNA fragment was subcloned into pCDNA3.1(+)vector. The results showed that the nucleic acid vaccine candidate pcDNA-FABP-NM has been constructed.
