Study of photosensitizers absorbed process by cells based on image processing technology
10.3760/cma.j.issn.1673-4181.2012.02.007
- VernacularTitle:基于图像处理技术分析细胞吸收光敏剂过程的研究
- Author:
Zhan SHU
;
Yingxin LI
;
Jichun YANG
;
Hongli CHEN
;
Xiaoxi DONG
- Publication Type:Journal Article
- Keywords:
Image processing;
Matlab;
Photosensitizer;
Fluorescence intensity
- From:
International Journal of Biomedical Engineering
2012;35(2):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the feasibility of analyzing photosensitizers absorbing process by cells based on image processing technology.MethodsFluorescence images of Laryngeal cells were taken by inverted fluorescence microscope after adding photosensitizers for a certain time.Parameter L that reflects the fluorescence intensity of cells in different times was obtained and the calculation results were compared.ResultsThe fluorescence intensity of cells increased over time.Sobel operater and Otsu algorithm can both reflect the fluorescence intensity of images.ConclusionImage processing technology can effectively analyzes the process of photosensitizers absorbed by cells.
