Effects of basic fibroblast growth factor pre-inducing on marrow stroma cells which were induced into dopaminergic neurons in vitro
- VernacularTitle:碱性成纤维细胞生长因子预诱导对骨髓基质干细胞向多巴胺能神经元分化的影响
- Author:
Huanyi CHEN
;
Ping NIU
;
Shuai ZHAO
- Publication Type:Journal Article
- Keywords:
basic fibroblast growth factor;
bone marrow stromal cells;
dopaminergic neuron
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of basic fibroblast growth factor(bFGF) pre-inducing on marrow stroma cells(MSCs) which were induced into dopaminergic neurons.Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats.MSCs were cultured,proliferated and purified by passage culture.After being induced by bFGF,cultuered MSCs were divided into experimental groups(GM1 group,GDNF group and GDNF+ GM1 group) and control group.The surface markers of the differentiated neuron,such as neurone specific enolase(NSE),glial fibrillary acidic protein(GFAP) and TH were detected by immunocytochemistry after MSCs were cultured in induction media for 3 days and 7 days.Results In control group,the NSE expression of MSCs was very low.Many NSE-positive cells and TH-positive cells were found in the experimental groups at 3 or 7 days after induction.The percentage of NSE-positive and TH-positive cells of GDNF+GM1 group was significantly more than the other experimental groups(all P
