A Study on Apoptosis of CL187 Colon Cancer Cells after ~(125)I Seeds Irradiation
- VernacularTitle:放射性~(125)I粒子照射CL187结肠癌细胞系凋亡的研究
- Author:
Hongqing ZHUANG
;
Junjie WANG
;
Hongxia ZHUANG
- Publication Type:Journal Article
- Keywords:
Radioactive seeds;
Apoptosis;
Colon cancer
- From:
Chinese Journal of Minimally Invasive Surgery
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of 125I seeds irradiation on apoptosis and cell cycle of CL187 colon cancer cells. Methods In vitro cultured human CL187 colon cancer cells were randomly divided into 4 groups:Control group,Irradiation groups under 2,5 and 10 Gy respectively,for which the activity of the seeds was determined at 9.25?104 MBq,and the initial dose to be 2.77 cGy/h.All the cells were collected 48 hours after irradiation.The apoptosis were detected by using double staining method with annexin and propidium iodide(PI),and the cell cycle by using PI staining method. Results The apoptosis rates were 13.74%?1.63%,46.27%?3.82%,32.58%?3.61% respectively in 2 Gy,5 Gy,and 10Gy groups,significantly higher than that of control group(1.67%?0.19%,q=7.594,P
