Establishing a trapezoid micro-plate method to detect ABO blood group in serum
- VernacularTitle:ABO血型反定型梯形微板法的建立
- Author:
Liping SUN
;
Lu HAN
;
Qingwu ZHANG
- Publication Type:Journal Article
- Keywords:
Red cell typing and serum ABO blood group typing;
Trapezoid micro-plate method;
U micro-plate method
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Establishing a trapezoid micro-plate method to detect ABO blood group in serum.Methods Select 32 blood samples containing A,B,O or AB types randomly.Use orthogonal design principle to select the optimum conditions of the blood plasma dilution,erythrocytes density,temperature and static time.The mix-blood samples are serially diluted.The agglutinating and nonagglutinating gray zones are also defined.Based on the optimum conditions and criterions,the reproducibility test,sensitiveness test,accuracy test,lipaemia interference test and haemolysis interference test are carried out.6 ABO subgroup(AM,AX,A2B,A3B,AXB,BM) and 5 irregular antibody(anti-M,anti-N,anti-P1,anti-Leb,anti-HI) are detected to evaluate the ability of the trapezoid micro-plate method to detect special samples.Results The trapezoid micro-plate method's repetitiveness tests coincidence is 100%.Its sensitivity is identical to the test tube method.The accuracy is 99.99%.In the 47 various kinds of lipaemia blood samples' anti-interference tests,the correct interpretation at one time is 100%.In the haemolysis interference tests,when hemoglobin content are below 20g/L,there are no obvious influences found.When sever haemolysis appears(blood plasma hemoglobin content are above 20g/L),there is no effect on the agglutination,but make a difference to the nonagglutinating.The ability to dectect 6 ABO blood subgroups and 5 irregular antibody blood samples is great.Conclusion The trapezoid micro-plate method not only has good ability to detect the ABO blood group both in the red cell typing and serum typing,but also enhances the accuracy in detecting the agglutinating blood samples and improves the exactitude of the tests.
