Granzyme B immunoreactivity in T/natural killer cell lymphomas.
10.3349/ymj.1997.38.5.285
- Author:
Min Sun CHO
1
;
Mi Kyung LEE
;
Woo Ick YANG
;
Joo Heon YOON
Author Information
1. Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
T/NK-cell lymphomas;
granzyme B;
immunohistochemistry
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Child;
Female;
Human;
Immunohistochemistry;
Killer Cells/pathology*;
Lymphoma/pathology*;
Lymphoma/enzymology*;
Lymphoma, T-Cell/pathology;
Lymphoma, T-Cell/enzymology*;
Male;
Middle Age;
Serine Endopeptidases/metabolism*
- From:Yonsei Medical Journal
1997;38(5):285-293
- CountryRepublic of Korea
- Language:English
-
Abstract:
Granzyme B is one of the serine proteases expressed in natural killer (NK) cells and activated cytotoxic T-lymphocytes. To evaluate the usefulness of granzyme B immunoreactivity in the diagnosis of T/NK-cell lymphoma, we studied 69 cases of lymphomas occurring in the upper aerodigestive tract by paraffin-section immunohistochemistry using a commercially available monoclonal antibody to granzyme B (GrB-7). All 19 cases of T/NK-cell lymphomas defined by the expression of CD56 (NHK-1) and one or both T-cell markers (polyclonal CD3 and CD45RO) expressed granular cytoplasmic granzyme B immunoreactivity. Two out of 9 cases of T-cell lymphomas showing no CD56 expression demonstrated strong granzyme B immunoreactivity. No tumor cells among 39 cases of B-cell diffuse large cell lymphomas and 2 cases of null cell diffuse large cell lymphomas were immunoreactive for granzyme B, however a few scattered granzyme B-positive reactive small lymphoid cells were consistently observed. Based on its sensitivity in this study as well as its reactivity in routinely processed tissue sections, even without heat-induced epitope retrieval technique, monoclonal antibody to granzyme B (GrB-7) can be applied as a useful marker in the diagnosis of T/NK-cell lymphomas in conjunction with CD56.
