Effects of propofol on apoptosis signaling pathways in renal ischemia/reperfusion injury of rat
- VernacularTitle:丙泊酚对大鼠肾缺血/再灌注损伤细胞凋亡信号通路的影响
- Author:
Jin LI
;
Shure LI
- Publication Type:Journal Article
- Keywords:
propofol;
renal ischemia/reperfusion;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a model of renal ischemic/reperfusion in rats and to observe whether propofol protects kidney from this injury, and to deduce a possible mechanism.Methods Ninty-nine male SD rats with right nephrectomy were randomly divided into three groups according to the different treatments to left kidney: Control group (n=26), IR group (n=35), IR+Pro group (n=38). The left kidney was exsected and blood samples were collected 0, 3, 12, 24, 72 h postsurgery respectively. Renal morphology dyed with HE was observed with light microscopy . Immunohistochemistry was used to detect the expression of apoptosis proteins including BCL-2、BAX、caspase 3、cytochrome C.Results Renal ischemia-reperfusion induced tissues injury, the severity of injury sequence was as follows: proximal convoluted tubule, distal convoluted tubule, collecting duct, renal corpuscle; According to the results of IHC, the expression of BAX, caspase 3, and cytochrome C in IR group were more than that in control group, and BCL-2 level was stable. The protective protein (BCL-2) level was higher and the proteins (BAX, caspase 3, and cytochrome C) inducing apoptosis were lower in IR+P group than that of IR group (P
