Treatment of Parkinson disease-like rat model by neural stem cell transplantation
- VernacularTitle:神经干细胞移植对帕金森病模型大鼠的治疗作用
- Author:
Min ZHENG
;
Yaping WANG
;
Xuetao PEI
- Publication Type:Journal Article
- Keywords:
neural stem cell, cell differentiation, Parkinson’s disease
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the survival, differentiation and action of neural stem cell transplanted into striatum of Parkinson disease rat model. Methods Neural stem cells were isolated and cultured. The character of self-renewing was confirmed by technique of single cell clone culture. The ability of multi-directional differentiation and specific antigen-nestin was showed by immunocytochemistry(ICC). PD rat models were established, and the DAPI-labelled neural stem cells were transplanted into striatum. The 6-HHDA induced rotary behavior was measured, distribution and migration of the cell were checked by fluoresce activating. ICC and HPLC are used to see its differentiation and action of secrete neurotransmitter dopamine(DA) and it’s metabolites. Results The cultured stem cell express nestin and have the ability of self-renewing and multi-directional differentiation. 6-HHDA induced rotation decreased after transplantation of NSC into the PD rat striatum(P
