Study on the extraction and purification of glycosaminoglycans from Rhopilema esculenta and its antihyperlipidemia effects
- VernacularTitle:海蜇糖胺聚糖提取、纯化及其降血脂作用研究
- Author:
Xiaoshi JIN
;
Hongmian WU
;
Min ZHONG
;
Xiaoling LEI
- Publication Type:Journal Article
- Keywords:
Rhopilema esculenta Kishinouye;
glycosaminoglycan(GAG);
extraction;
purification;
anti-hyperlipidemia
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the extraction and purification of glycosaminoglycans from Rhoqilema esculenta Kishinouye,optimize the enzymolysis process,and its antihyperlipidemia effects were studied.Methods Extraction of glycosaminoglycans(GAG) from Rhopilema esculenta Kishinouye by hydrolyzation method with papain,subtilisin and trypsin neutral proteinase,ethanol precipitation.The rude product of glycosaminoglycans(GAG) was purified and fractionated by the methods of adsorption,dialysis,ethanol precipitation and CTAB precipitation.The experimental hyperlipidemia mice induced by high fat forage were studied.The total cholesterol(TC) and triglyceride(TG) levels were determined.Results Optimum enzymolysis process: papain neutral proteinase1.5%,subtilisin neutral proteinase1.5%,treated for 5h;The result showed that the levels of TC and TG decreased to 6.83?1.09 mmol?L-1(P
