Effects of LaCl3 on P16 and P21 expressions of hepatocellular carcinoma cells
- VernacularTitle:三氯化镧对肝癌细胞P16和P21蛋白表达的影响
- Author:
Wenhua JIANG
;
Dong CHEN
;
Liming HAO
;
Xiaoting MENG
- Publication Type:Journal Article
- Keywords:
lanthanoid series elements;
lanthanum trichloride;
tumor cells,cultured;
liver neoplasms;
cell cycle;
P16;
P21
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of LaCl3 on P16 and P21 expressions of hepatocellular carcinoma cells.Methods The experiment was divided into 4 groups.In experiment groups,CBRH-7919 cells were cultivated with 0.01,0.10 and 1.00 mmol?L-1 LaCl3 in DMEM containing 10% calf serum.In control group,CBRH-7919 was cultivated in DMEM containing 10% calf serum without LaCl3.The growth of CBRH-7919 cells was observed following treated with 0.01,0.10,1.00 mmol?L-1 LaCl3 for 1,3,5 d in vitro, respectively,the changes of cell cycle were detected by flow cytometry.At the same time,P16 and P21 were detected by immunocytochemical analysis.Results Compared with control group,the inhibitory rate of growth of CBRH-7919 cells was increased evidently when treated with 0.1 and 1.00 mmol?L-1 LaCl3 for 3 and 5 d(P
