Effect of antioxidant PDTC on expression of IL-8 in human ovarian caner cell line SKOV3 in acidic environment
- VernacularTitle:抗氧化剂PDTC对酸性环境诱导的人卵巢癌SKOV3细胞IL-8表达的作用及机制
- Author:
Kun WANG
;
Manhua CUI
;
Kai HE
- Publication Type:Journal Article
- Keywords:
ovarian neoplasms;
interleukin 8;
NF-kappaB;
pyrrolidine dithiocarbamate
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pyrrolidine dithiocarbamate (PDTC) on the proliferation and expression of IL-8 of SKOV3 cells in acid environment,and the role of nuclear factor ?B (NF-?B) in the process.Methods SKOV3 cell lines were divided into 5 groups: normal culture group,acid environment group,acid environment plus PDTC groups (25,50 and 100 mol?L-1 PDTC).All the SKOV3 cells were cultivated for 12 h.The effect of PDTC on the proliferation of SKOV3 in the acid environment was determined by MTT,the content of IL-8 protein in the culture supernatant was detected by ELISA and the expression of NF-?B protein was measured by Western blotting.Results The IL-8 expression level in the acid environment was(1 384.211?42.320) ng?L-1.there was significant difference compared with normal group(617.505 ng?L-1?47.850 ng?L-1)(P
