Effects of OHAP-1 on expression of bcl-2/bax mRNA and oxidative stress in rat C6 glioma cells
- VernacularTitle:OHAP-1对大鼠C6胶质瘤细胞bcl-2/bax基因表达和氧化应激的影响
- Author:
Zhanpeng ZHU
;
Jinsong KANG
;
Xiaoxia KONG
;
Yinan LUO
;
Liankun SUN
- Publication Type:Journal Article
- Keywords:
Okinawa Habu apoxin protein-1;
glioma;
apoptosis associated gene;
oxidative stress
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Okinawa Habu apoxin protein-1 (OHAP-1) on the proliferation inhibition of rat C6 glioma cells and its mechanisms. Methods MTT colorimetric analysis was used to measure the inhibitory effect of OHAP-1 with different doses(2.5,5.0,and 10.0 mg?L-1) on C6 glioma cells .RT-PCR was used to evaluate the mRNA expressions of bcl-2 and bax genes.The activity of superoxide dismutase (SOD) and the level of maleicdialdehyde (MDA) in the C6 glioma cells were also examined. Results The proliferation of C6 glioma cells was significantly inhibited by different doses of OHAP-1(2.5,5.0,and 10.0 mg?L-1).The inhibitory rate were 49.77%,67.65%,and 76.42%,respectively.The inhibitory rate in 2.5,5.0, and 10.0 mg?L-1 groups were higher than that in control group(P
