Influence of PDTC on expression of LFA-1 mRNA induced with LPS in mouse non-specific keratitis reaction
- VernacularTitle:非特异性角膜炎症反应中PDTC对LPS诱导LFA-1 mRNA表达的影响
- Author:
Guangheng WU
;
Jiaying ZHANG
;
Lihong YANG
;
Le ZHOU
- Publication Type:Journal Article
- Keywords:
leukocyte function-associated antigen-1;
NF-?B;
keratitis;
subconjunctival injection;
pyrrolidine dithiocarbamate;
lipopolysaccharides
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between leukocyte function-associated antigen-1 (LFA-1) mRNA expression and corneal inflammation and discuss the inhibition reaction of nuclear factor-?B(NF-?B)inhibitor on LFA-1 mRNA expression.Methods The corneal suture or combined with subconjunctival injection models were constructed in BALB/C mice.The corneas of each group were excised 1,3,7 and 14 d after operation and LFA-1 mRNA expressions were investigated by reverse transcription polymerase chain reaction(RT-PCR).Results At 1,3 and 7 d after operation, the expressions of corneal LFA-1 mRNA in corneal suture combined with lipopolysaccharide (LPS) (corneal suture+LPs)subconjunctival injection group were higher than those in corneal suture group (P0.05).At 1 and 7 d after operation, compared with corneal suture+LPS group ,the expression of corneal LFA-1 mRNA increased,but it decreased at 3 and 14 d after operation in the group of corneal suture combined with LPS and pyrrolidine dithiocarbamate (PDTC) (P
