Mechanotransduction in Differentiation of Osteogenic From Mesenchymal Stem Cells
- VernacularTitle:机械力对间充质干细胞向成骨细胞分化的力学响应机制研究
- Author:
Hongbin ZHAO
;
Tongde LU
;
Jing MA
;
Hui MA
;
Xizheng ZHANG
- Publication Type:Journal Article
- Keywords:
strain, mesenchymal stem cells, osteoblast, Ca2+, strain signaling
- From:
Progress in Biochemistry and Biophysics
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the effect of mechanical strain on mesenchymal stem cells (MSCs) differentiation into osteogenic, the cyclic substrate deformation instructment was applicated to MSCs line (D1 cell) in osteogenic media for different strain and times. The cells were cyclically stretched for periods of 1, 2, 3, 5 and 10 min and treated with cytochalasin B(CB) and EGTA. Ca2+, which were loaded with (15 ?l) Fluo-3 AM , were detected by confocal laser scanning microscope(CLSM). The signaling inhibitors, such as SB203580(p38MAPK specific inhibitor), PD98059(MEK-1/2MAPK specific inhibitor), LY294002(PI3Ks specific inhibitor), cytochalasin B(microfilament specific inhibitor), EGTA (Ca2+ specific inhibitor), were used to investigate mechanical signaling pathway by (3% 0.5Hz) Cyclic strain. The results indicated that 3% strain stimulus could induce increasement of [Ca2+] i - dependent fluorescence at 2 min. Its fluorescence intensity were 143.68,which were significantly different compared with 1,3,5,10min groups and 1% and 10% strain groups (P
