Effect of stem cell factor antisense oligonucleotide on the interaction of fibroblasts and murine mast cells
- VernacularTitle:干细胞因子反义寡核苷酸对小鼠成纤维细胞-肥大细胞相互作用的影响
- Author:
Ruilin LIU
;
Wenzhuo YANG
;
Qiang LI
;
Hanjing L
;
Weili WEI
;
Min ZHANG
- Publication Type:Journal Article
- Keywords:
asthma;
mast cells;
fibroblast;
stem cell factor;
antisense oligonucleotide
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of stem cell factor(SCF)-mediating fibroblasts and mast cells interaction in pathophysiology process of asthma.Methods We transfected NIH3T3 cells with SCF antisense oligonucleotide(SCF ASON) and detected SCF expression by immunochemistry and RT-PCR.Then,we isolated mast cells from mouse bone marrow and established NIH3T3 and mast cells cocultures.After SCF ASON intervention,histamine and eotaxin levels in culture supernatants were determined by ELISA and fluorometry.Growth curves of fibroblasts and mast cells were drawn.We also observed mast cells apoptosis by AO stain and flow cytometric analysis.Results SCF ASON strikingly down-regulated SCF protein and mRNA level of NIH3T3 cells.SCF ASON intervention inhibited the growth of NIH3T3,and induced mast cell apoptosis in cocultures(14.0%?0.81% at 96 hour).Both histamine[(3.08?0.38)?g/L vs(3.83?0.41)?g/L,P
