SELEX and its applications in medicine
- VernacularTitle:SELEX技术及其在医学中的应用
- Author:
Yan WANG
- Publication Type:Journal Article
- Keywords:
SELEX;
aptamer;
ligand;
oligonucleotide;
library
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Systematic evolution of ligand by exponential enrichment(SELEX) is a new combinational chemical methodology for in vitro selection of specific aptamers.Aptamers are artificial oligonucleotide ligands with high-affinity binding to target molecules,such as amino acids,drugs,proteins and other small molecules or ions and so on.They are isolated from combinational libraries of synthetic oligonucleotide by an iterative process of affinity selection,recovery and amplification.Several properties of aptamers make them an attractive class of therapeutic compounds.Their affinity and specificity for a given protein make it possible to isolate a matching ligand,and to adjust their bioavailability.Aptamers may be useful in the treatment of a variety of human diseases including infectious illness,cancer and cardiovascular diseases.The development of this emerging class of therapeutics is reviewed,in terms of progress,and potentially clinical application.
