Screening of alginate producing strain and optimization of fermentation condition
- VernacularTitle:褐藻酸降解菌的筛选及产酶条件优化
- Author:
Min PANG
;
Changhong WANG
;
Baoqin HAN
;
Wanshun LIU
- Publication Type:Journal Article
- Keywords:
alginase;
optimization of fermentation condition;
enzyme characterization
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A strain with high alginase activity was screened and isolated by decomposing sodium alginate from the decaying parts of brown alga Laminaria japonica and Undaria pinnatifida,in order to produce alginase.Methods The strain s4 with high alginase activity was chosen by filtration.The alginase producing media was optimized and the alginase was produced and its characterization was investigated.Results The optimum fermentation conditions for alginate lyase producing as follows: media AlgNa 1.2%,NH4Cl 0.9%,NaCl 1.5%,pH=7.5,and temperature 25℃.Conclusion The alginase produced by strain s4 showed high alginase activity and good stability.
