Effect of cyclopamine on growth and proliferation of human mammary carcinoma cell Bcap-37
- VernacularTitle:Cyclopamine对人乳腺癌细胞Bcap-37生长和增殖的影响
- Author:
Haibo WANG
;
Yali LIU
;
Yuzhen HU
;
Sumin CHI
- Publication Type:Journal Article
- Keywords:
Hedgehog;
Cyclopamine;
Breast cancer;
Cell line Bcap-37;
Cell proliferation
- From:
Journal of Medical Postgraduates
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of cyclopamine on growth and proliferation of human mammary carcinoma cell line Bcap-37.Methods:The Bcap-37 cells were incubated with cyclopamine,which is the specific inhibitor of Hedgehog signaling pathway.Then the inverted microscope was used to observe the morphologic changes of the cells,and MTT assay,BrdU incorporation and cell cycle analysis were used to examine the influence of cyclopamine on Bcap-37 cell growth and DNA synthesis.Results:Compared with the control group,after the cells were incubated with cyclopamine,obvious morphologic changes of Bcap-37 cells were observed;the growth of Bcap-37 cell was inhibited in a dose and time dependent manner;the DNA synthesis of Bcap-37 cells was obviously inhibited;the number of Bcap-37 cells in S phase decreased and the cells were blocked in G2 phase.Conclusion:Cyclopamine can effectually inhibit the growth and proliferation of Bcap-37 cells,which indicates that Hedgehog signaling pathway may be inappropriately activated in breast cancer and inhibiting Hedgehog signaling pathway can be a useful method in breast cancer treatment.
