Inhibition of phyticacid and bivarudin on platelets activation in vitro
- VernacularTitle:植酸钠和百维利肽体外抑制血小板活化的实验研究
- Author:
Jinghan LIU
;
Jun ZHOU
;
Dongmei WANG
- Publication Type:Journal Article
- Keywords:
Phyticacid;
Bivarudin;
Platelet activation;
Platelets preservation
- From:
Chinese Journal of Blood Transfusion
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To select the reversible activation inhibitors for platelet retreating before lyophilization.Methods Effects of phyticacid and bivarudin of different concentration on platelets in vitro viability and functional activity were evalued by the maximal aggregation of platelets,CD62p and PAC-1 expressions and re-expressions stimulated by thrombin,and platelets SPAT.Results Expression of CD62p and PAC-1 increased after treatment.Phyticacid with a concentration of 1mmol/L could decline the PAC-1 expression,but could not decline the re-expression of CD62p and PAC-1,and the aggregation reaction induced by propylgallate was also maintained.Bivarudin at a concentration of 1mmol/L could inhibit both expressions(0.78% and 0.24%) and re-expressions(7.00% and 14.39%) of CD62p and PAC-1.Aggregation reactions stimulated by inducers except thrombin were maintained.With phyticacid concentration ≤2mmol/L,the aggregation time was prolonged;while the aggregation time was the same with ≤ 3?mol/L bivarudin.Conclusions Phyticacid with the concentration of 1mmol/L could inhibit the GPIIb/IIIa conformation change of platelet,while 1?mol/L Bivarudin could inhibit platelet activation,and a better protection will be observed with a lower concentration.
