Screen of human single chain antibody against N protein of SARS-CoV
- VernacularTitle:抗SARS-CoV病毒N蛋白的单链抗体(scFv)筛选
- Author:
Zhenyan SHI
;
Bin YIN
;
Qun WEI
;
Xiaozhong PENG
- Publication Type:Journal Article
- Keywords:
SARS-CoV, nucleocapsid protein, phage display library of human single chain antibodies
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the single chain antibody against N protein of SARS-CoV. Methods With N protein of SARS-CoV expressed in E.coli as antigen, we obtained the single chain antibody against N protein by screening the phage display library of human single chain antibodies. Results The anti-N protein antibody didn’t cross-interacte with BSA and the short peptide containing 6 histidines. The specific interaction between the antibody and N protein was inhibited by the anti-N protein monoclone antibody from immunized mice. ConclusionThe single chain antibody we got is specific to N protein of SARS-CoV,it can be a candidate antibody for fast detection of N protein of SARS-CoV and SARS virus particles in clinical trial study of SARS pathogenesis.
